XXIII Congress of International Society for Molecular Plant-Microbe Interactions


 

El Instituto de Biotecnología y Biología Molecular (IBBM / CONICET), celebra la invitación realizada por la International Society for Molecular Plant-Microbe Interactions a la Dra. María Eugenia Zanetti, destacada investigadora de nuestra institución, para participar como miembro disertante del trabajo titulado “Unrevealing the translational reprogramming of root cells during nitrogen fixing symbiosis” durante el XXIII Biennial Congress on Molecular Plant-Microbe Interactions, en la Ciudad de Glasgow, Escocia, del 14 al 18 de Julio de 2019.
El programa del Congreso incluye temas relacionados con reconocimiento molecular en inmunidad de plantas, manipulación microbiana del huésped, sistemas emergentes y reemergentes, entre otros asuntos de gran interés en la actualidad.

UNREVEALING THE TRANSLATIONAL REPROGRAMMING OF ROOT CELLS DURING NITROGEN FIXING SYMBIOSIS
Abstract:
Legume plants have acquired the ability to establish a nitrogen fixing symbiosis with rhizobial bacteria. The establishment of this symbiotic interaction requires the formation of a new lateral root organ, the nodule, and the suppression of the immune response that will allow rhizobia to infect the root tissue. The activation and coordination of these two processes require the reprogramming of root cells for symbiosis, which is accompanied by dramatic alterations in gene expression. Regulation of gene expression occurs at different tiers, including transcriptional, co-transcriptional and post-transcriptional events. Our studies have been focused on the co- and post- transcriptional events, including alternative splicing, translation and non-coding RNA-mediated repression. By combining TRAP (Translating Ribosome Affinity Purification) with Ribosome footprinting, we have profiled the root translatome at early stages of the legume-rhizobia symbiosis. Our results reveal that translation of hundreds of transcripts is finely regulated by rhizobia and that translational alterations are not necessarily correlated with changes in transcript abundance. Transcripts with altered translational status include protein-coding and non-coding RNAs, some of which exhibited cell-specific regulation. Transcript variants produced by either alternative splicing or alternative cleavage and polyadenylation are also tightly regulated at translational level. Using molecular and genetic approaches we have revealed that these translationally regulated transcripts play crucial roles in the establishment of a successful nitrogen fixing symbiosis.

Lista de Autores:
Soledad Traubenik
Instituto de Biotecnología y Biología Molecular, UNLP and CCT La Plata-CONICET
Mauricio A Reynoso
Instituto de Biotecnología y Biología Molecular, UNLP and CCT La Plata-CONICET
Karen V Hobecker
Instituto de Biotecnología y Biología Molecular, UNLP and CCT La Plata-CONICET
Milagros Ferrari
Instituto de Biotecnología y Biología Molecular, UNLP and CCT La Plata-CONICET
Maureen Hummer
Center for Plant Cell Biology, University of California, Riverside
Julia Bailey-Serres
Center for Plant Cell Biology, University of California, Riverside
Flavio A. Blanco
Instituto de Biotecnología y Biología Molecular, UNLP and CCT La Plata-CONICET
Maria Eugenia Zanetti
Instituto de Biotecnología y Biología Molecular, UNLP and CCT La Plata-CONICET

https://ismpmi.confex.com/ismpmi/2019/meetingapp.cgi/Paper/1292